Figure 3.
Interaction between synaptically released glutamate and bath-applied kainate. A, Summary histogram showing the relation between the number of synaptic stimuli and the inhibition of IsAHP. B, Summary time course of IsAHP inhibition recorded during 200 nm kainate application, followed by 5 pulse, 100 Hz stimulation (n = 6). Kainate application occluded the inhibitory action of synaptic glutamate release. C, Summary data from six experiments. No occlusion was observed when 200 nm kainate was added after a five-pulse tetanus. D, Summary time course of IsAHP inhibition recorded after 20 pulse stimulation, followed by 200 nm kainate application from six experiments. In this case tetanic stimulation did occlude the action of subsequent kainate application. E, Histogram to compare the combined effects of a 5- or 20-pulse train in combination with kainate application. All panels show mean ± SEM.