Figure 1.
Blocking GAT-1 enhances stimulus train-induced slow GABAAR-mediated currents in neonatal CA3 neurons. A, Specimen voltage-clamp recordings at a holding potential of 0 mV with a low chloride pipette filling solution showing currents evoked by a train of stimuli (9 pulses, 10 Hz) indicated by the horizontal bar shown at the top (stimulus artifacts are truncated). All recordings were done in the presence of 10 μm NBQX, 40 μm d,l-AP-5, and 1 μm CGP. NO-711 was applied at 2.5 μm, and bic was applied at 10 μm. B, Bar graph showing the duration (defined as time of decay to 30% from the instantaneous level) of the slow current at the time of the last stimulus pulse and the mean total charge transfer after the last pulse (n = 6 recordings). Events occurring >4 min after the start of NO-711 application were included in the quantification. C, Voltage-clamp recordings showing currents evoked by a train of stimuli (30 pulses, 10 Hz; horizontal bar) in a solution containing 0.5 mm Ca2+ plus 5 mm Mg2+ (top trace), with 2.5 μm NO-711 (middle trace), and subsequent addition of 1 μm TTX (bottom trace).