Figure 1.
Signaling pathways activated by CNTF in the mouse retina. Western blots of STAT and ERK proteins as results of growth factor stimulations are shown. A, Phosphorylation of STAT1, STAT3, and ERK in dissociated P0 mouse retinal cells treated with BDNF, LIF, CNTF, or EGF (all at 10 ng/ml) for 15 min. The top panels were probed with anti-phospho-STAT1 (pSTAT1), phospho-STAT3 (pSTAT3), or phospho-ERK (pERK) antibodies. The bottom panels show the levels of total STAT1, STAT3, or ERK1/2 in the same cell extracts, respectively. B, Time courses of phosphorylation for STAT1, STAT3, and ERK in dissociated P0 mouse retinal cells after incubation with CNTF (10 ng/ml). The levels of total STAT1, STAT3, and ERK proteins are shown in the bottom panels. C, Phosphorylation of STAT3 or ERK in dissociated P3, P7, and P10 retinal cell cultures after 15 min stimulation with CNTF (10 ng/ml) or EGF (10 ng/ml). The bottom panels show total STAT3 or ERK proteins in the same extracts. Western blots shown represent one of three independent trials, which gave similar results.