Figure 2.
Immunoperoxidase staining for cortactin. A, Low-magnification view of a parasagittal section of adult rat brain. Staining is especially prominent in the cerebellar cortex and hippocampus and is also strong in other brain regions rich in dendritic spines. B, Micrograph showing the hippocampal formation. Staining is conspicuous in the molecular layer of the dentate gyrus (DG) and in the neuropil of CA1 (dashed lines mark borders of the main regions within the hippocampal formation). C, D, CA1 of hippocampus, showing stained somata and apical dendrites of pyramidal neurons. The detail in C reveals patchy staining in the somatodendritic compartment, surrounding immunonegative nuclei. E, High-magnification view of the stratum radiatum reveals intensely labeled dendrites; puncta in the neuropil may correspond to dendritic spines. Scale bars: A, 5 mm; B, 500 μm; C, E, 25 μm; D, 50 μm.