Figure 3.
Role of the Nogo receptor in axon regeneration. A-F, Longitudinal sections through the optic nerve showing GAP-43-positive axons distal to the injury site (asterisk) 2 weeks after surgery with or without lens injury. A-C, Axon regeneration after transfection of growth-activated RGCs with AAV expressing GFP alone (A), NgRWT (B), or NgRDN (C). D, NgRDN expression fails to increase regeneration when RGCs have not been sensitized to grow. E, F, Distal optic nerve from the NgRDN-expressing, growth-sensitized case shown in C. Many of the longest axons coexpress GAP-43 (E) and GFP (F) (arrows). Scale bars: A-D, 200 μm; E, F, 100 μm.