Figure 6.
Reduced sensitivity to nociceptive stimuli in OSM-/- mice. A-D, Tests of chemical nociception. A, Duration of licking in response to intraplantar injection of capsaicin (1.0 μg; n = 6) is significantly reduced in mutant mice (black columns) compared with wild-type (white columns) and heterozygous mice (gray columns); the last two groups do not differ. B, Duration of licking in response to capsaicin at indicated doses (n = 5). C, Duration of licking in response to αβ-meATP (n = 6). D, Cumulative time spent licking the hindpaw after the subcutaneous injection of formalin (n = 6). Phase I, 0-10 min after injection; Phase II, 15-50 min after injection. E, Licking or jumping latency in the hot-plate assay (n = 6). F, Response latency to acute mechanical stimulus by tail clip (n = 6). G, H, Visceral pain responses (writhing) produced by the intraperitoneal injection of MgSO4 (G) or acetic acid (H) (n = 8-9 for both wild-type and mutant). NS, Not significant. *p < 0.05; **p < 0.02; ***p < 0.01; ****p < 0.001.