Figure 3.
Effect of VILIP-2 on facilitation and inactivation of CaV2.1 channels during trains of repetitive depolarizations. Test pulses to +10 mV (0 mV for IBa) for 5 ms at a frequency of 100 Hz were applied to transfected tsA-201 cells expressing CaV2.1 channels only (gray) or CaV2.1 channels plus VILIP-2 (black). Peak current amplitudes were normalized to the first pulse in the series and plotted against time (±SEM; n ≥ 10; every 10th SEM is plotted). A, Representative ICa measured for the first pulse, the pulse at 355 ms, and the last pulse in a train of stimuli for CaV2.1/β2a alone. B, Representative ICa measured for the first pulse, the pulse at 355 ms, and the last pulse in a train of stimuli for CaV2.1/β2a plus VILIP-2. C, ICa, CaV2.1/β2a. D, ICa, CaV2.1/β1b. E, IBa,CaV2.1/β2a. F, IBa, CaV2.1/β1b. Error bars represent SEM.