Figure 2.
Examples of PNs from females (A–F) and males (G–L) with dendritic arborizations confined to G35 [described by Rospars and Hildebrand (1992, 2000)].A–D,G–K,Confocal stacks collected from whole-mount preparations or after embedding in plastic and sectioning. In both sexes,G35-PNs have their somata in the anterior group of AL neuronal cell bodies (not visible in these figures; see Results)and an axon (Ax) projecting from the AL. C, H, J, Higher-magnification images of female (C) and male (H, J) G35-PNs after sectioning (C and H are from the PNs shown in A and G,respectively).These figures illustrate the fine and dense dendritic arborizations of G35-PNs and the close anatomical relationship between G35 and the LFGs (latLFG and medLFG are the lateral and medial LFGs, respectively)in females (C)or the MGC (C and T1 are the principal glomeruli of the MGC, Cumulus and Toroid 1,respectively) in males(H,J).D,K,Axonal projections of the PNs shown in B(female) and G(male), showing collaterals in the calyces of the ipsilateral mushroom body (CMB)(dottedlines) and terminals in the lateral horn (LH) of the protocerebrum (arrows). Note that the morphological features and projection sites in the protocerebrum of G35-PNs are indistinguishable in males and females. E, F,L,3D reconstructions (frontal view) of ALs from three brains with physiologically and morphologically characterized PNs from females (E,F) and from a male(L). These reconstructions show that, in both sexes, G35 (which houses the dendritic arborizations of the stained PNs, in black) occupies the same relative position with respect to the other glomeruli of the AL.The latLFG (E,F)(the medLFG is not visible in this view) and the T1 and C(L) are shown in gray. One, three, and two anterior glomeruli are omitted (E,F, and L, respectively) for visualization of G35.d, Dorsal; l,lateral.Scalebars: A,B,D,G,I,K, 100 μm; C,H,J, 50 μm.