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- supplemental material - Figure S1: Interpretation of the role of RTN. a: under anesthesia, the intrinsic response of RTN neurons is a linear function of pH and these neurons probably derive a considerable portion of their on-going activity from their intrinsic chemosensitivity given the modest effect of glutamate receptor blockade with KYN. RTN neurons lack intrinsic bursting properties and activate the CPG via still unknown targets. The CPG, in turn, exerts a feedback on RTN neurons causing the respiratory modulation observed in vivo and the saturation of the response of both RTN neurons and PND at high levels of CO2. b: the feedback of the CPG on RTN neurons probably involves inhibitory inputs from each of the four classes of inhibitory neurons involved in generating the inspiratory motor outflow. The medullary network responsible for generation of the inspiratory motor outflow is redrawn after Rybak et al., 2004.
- supplemental material - Figure S2: Location of glutamatergic and GABAergic neurons in the RTN region. Vesicular glutamate transporter 2 (VGLUT2) mRNA and glutamic acid decarboxylase 67 (GAD67) mRNA were detected by in situ hybridization in adult rat brain slices as described in Weston et al., (2004). This figure shows that RTN is a predominantly glutamatergic nucleus and that the region located rostral and lateral to RTN below the facial motor nucleus (the latter identified by the number 7) contains few if any neurons. A: VGLUT2 mRNA at level -11.6 mm relative to Bregma according to the atlas of Paxinos and Watson (1998). This level corresponds to the caudal end of the facial motor nucleus. The RTN contains numerous VGLUT2 mRNA-positive neurons. B: more rostrally (level -11.3 mm of the atlas of Paxinos and Watson), the region located below the lateral half of the facial motor nucleus is virtually devoid of VGLUT2-expressing neurons except for a very few cells within the marginal layer (arrows). C, D: neither RTN not the region below the lateral half of the facial motor nucleus contain significant numbers of GAD-67 expressing neurons (arrow in D points to a single cell located in the marginal layer). Calibration 500 ?m in D applies to all panels.