Figure 3.
Presence of PP2cα in presynaptic boutons in hippocampal neurons. A, Immunostaining of a presynaptic protein, synapsin I, in GFP-PP2cα-transfected neurons. B, Green fluorescence of the same neuron, exhibiting punctate distribution of the GFP-tagged PP2cα.C, Overlay of the red (synapsin I) and green fluorescent (GFP-PP2cα) images. D, Synapsin I staining of a neuron transfected with GFP alone. E, Green fluorescence of the same neuron, exhibiting uniform distribution of GFP. The fluorescence on the cell body was saturated. F, Overlay of the red (synapsin I) and green fluorescent (GFP) images.