Figure 1.
Reduced rate of spontaneous action potential firing in Kv3.3 KO Purkinje neurons. A1, Single-unit potentials were recorded from the cell bodies of a WT (top trace) and a Kv3.3 KO Purkinje neuron (bottom trace). The spike rates are 27.2 ± 0.9 Hz (WT) and 18.3 ± 0.8 Hz (Kv3.3 KO), close to the ensemble means given in B. A2, Distribution of instantaneous spike frequency of cells shown in A1. The histograms fit to the normal distribution with a correlation of R > 0.98. The widths of both distributions are not different (WT, σ/μ = 0.035 ± 0.005; Kv3.3 KO, σ/μ = 0.038 ± 0.008). B, Median spike rates measured in WT (61 cells, 11 animals, 25–51 d old; open columns) and Kv3.3 KO (52 cells, 7 animals, 30–51 d old; filled columns) Purkinje neurons at 24 ± 0.5°C. C, Spontaneous firing rate as a function of bath temperature. Columns indicate means ± SEM of firing rates measured at 25, 30, and 35°C (± 0.5°C) from WT (4 cells) and Kv3.3 KO (5 cells) Purkinje neurons. All measurements were performed in ACSF containing NBQX (20 μm) and picrotoxin (50 μm).