Figure 7.
Immunohistochemical localization of the AT1R in the DMH of an adult male rat. A, Western blotting of the different dilutions of the AT1R with different concentrations of rat brain lysate. B, Western blotting for the AT1R in rat brain lysate after previous incubation of the AT1R antibody without (control) and with a 5×, 10×, and 20× concentration of an AT1AR and AT1BR mixture. C, Illustration of the a rat brain section (−3.14 mm from bregma) containing the DMN, dorsal hypothalamic area (DA), LH, median eminence (ME), and PeF, adapted from a standard stereotaxic rat brain atlas. The gray-filled rectangle represents the DMH, which is a region that is particularly sensitive to BMI-induced cardioexcitatory response (Samuels et al., 2004) and receives projections from CVOs such as the OVLT (Thompson and Swanson, 1998) and contains the DMN, dorsal hypothalamic area, and PeF. Boxes with gray lines represent regions photograph (D, E, dorsal hypothalamic area; F, LH; G, median eminence). High-magnification photographs of AT1R-immunopositive cells in the rat dorsal hypothalamic area (D, E), LH (F), and median eminence (G). High-magnification photograph of the AT1R-immunopositive cells in the DMH with a 1:500 concentration of AT1R antibody without (I) and with (J)10 × concentration of an AT1AR and AT1BR mixture. Scale bar: D, 100 μm; E, F, 50 μm; bottom right insets in E, F, 17 μm; G, H, 42 μm; I, 50 μm. 3V, Third ventricle; f, fornix; mfb, median forebrain bundle; mt, mammillothalamic tract; opt, optic tract.