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Supplemental data
Files in this Data Supplement:
- supplemental material - Table 1
- supplemental material - Table 2
- supplemental material - Table 3
- supplemental material - Supplementary Figure 1. Distribution of the durations of the epileptiform events recorded extracellularly in 44 slices in 0 extracellular Mg2+ and 50-100 μM picrotoxin (A, bin 0.5 s) and from 17 slices in 0.5 mM Mg2+ and 8.5 mM K+ (B, bin 50 ms).
- supplemental material - Supplementary Figure 2. SICs detected in recordings from pyramidal neurons of the area CA3 of the hippocampus. SICs were observed in area CA3 with a variety of conditions that stimulate glutamate release from astrocytes including slice perfusion with low extracellular Ca2+.
- supplemental material - Supplementary Figure 3: SICs and epileptiform activity: two pharmacologically and spatially distinct phenomena. A) Simultaneous whole cell recording from a CA1 pyramidal neuron and two extracellular recording from CA1 and CA3 regions as depicted in the inset on the left. Ictal-like activity is synchronous in all the electrodes while SICs (red asterisk) recorded from the whole cell electrode are absent in extracellular distal electrodes. Inset on the bottom shows an example of ictal-like event and an SIC. Unclamped action potential in the whole-cell electrode have been truncated for presentation purposes. B) In the same experiment shown in A) application of TTX abolished the epileptiform activity but not SIC currents. See inset for traces at expanded time scale.
- supplemental material - Supplementary Movie 1 legend. Synchronous Ca2+ oscillations in groups of hippocampal CA3 pyramidal neurons. Cytosolic Ca2+ levels of hippocampal pyramidal neurons are shown in a pseudocolor display depicting estimated internal Ca2+ levels using experimental approaches detailed for Figure 2. Successive sequences show a CA3 region first, at basal conditions, second, following slice perfusion with saline containing 0 Mg2+ and picrotoxin in the absence of TTX, and, lastly, following addition of TTX. Perfusion with saline containing 0 Mg2+ and picrotoxin in the absence of TTX induces Ca2+ oscillations that are synchronized across all neurons. These events represent neuronal discharges during epileptiform activity. Following addition of TTX (0.5 μM) while epileptiform activity is blocked, spatially restricted synchronous neuronal Ca2+ elevations, that are evoked by glutamate released from astrocytes, can still be observed. Repetitive Ca2+ elevations in an astrocyte (cell a in Fig. 2D, image 1) can be also observed. Time stamp on movie is in seconds.