Figure 1.
Acute Aβ impairment of mitochondrial transport in cultured neurons. a, Representative images in differential interference contrast of hippocampal neurons (left panels), their mitochondria (middle panels), and the movement traces (right panels) before (top row) and after (bottom row) 30 min Aβ25–35 treatment. For clarity, the fluorescent images were displayed in reversed grayscales. Scale bars, 10 μm. b, Percentages of moving mitochondria remaining after 30 min treatment of 3, 7, and 14 DIV cells with control saline (Control) and Aβ25–35. c, Dose dependence of Aβ25–35 inhibition of mitochondrial transport. The reserve peptide Aβ35–25 (20 μm) was the control. d, Time course of Aβ25–35 inhibition of mitochondrial movement in 3 DIV hippocampal cells. e, Exposure time of Aβ25–35 required for inhibition of mitochondrial transport. Cells (3 DIV) were incubated with 20 μm Aβ25–35 for various periods of time, followed by washout and imaging before and 45 min after the beginning of Aβ application. Ctrl, Control; MC, mitochondria. Error bars indicate SEM. *p < 0.01, significant difference from corresponding control (Student's t test).