Figure 1. A, Sagittal frozen sections through the lesion site demonstrate the distribution of transplanted GFP-OEC. Transplanted cells are primarily confined to the lesion site. Some cells migrated into the deep white matter. The dashed line demarcates lesion edge. B, Coronal frozen sections in the lesion show the presence of GFP-OEC within a lesion site. Transplanted cells survived primarily in the dorsal funiculus. There was little GFAP staining within the lesion zone. C, D, H, GFAP-positive cells were present at the peripheral margin of the lesion. These results indicate that few astrocytes are present in the transplant region, and that there is a preponderance of GFP-OEC in the lesion zone. E, High-magnification micrographs of semithin plastic sections stained with methylene blue/Azure II through the OEC transplanted lesion demonstrated that the transplanted dorsal funiculus was extensively myelinated. F, P0-immunostaining of the frozen coronal section reveals that most axons remyelinated by transplanted OECs are surrounded by a peripheral type of myelin. F, G, Peripheral roots are strongly immunostained by P0 (arrowheads). I, Red-P0 rings are associated with green cellular elements, indicating that transplanted OECs remyelinate the demyelinated axons. I, Inset, Expansion of a cell indicated by an arrow. Scale bars: A, 1 mm; (in G) B–D, F, G, 400 μm; E, 10 μm; H, 30 μm; I, 10 μm; A, inset, 20 μm; B, inset, 10 μm.