Figure 6.
Effect of MTS compounds on ATP potency at cysteine mutants. A, At N290C, MTSES decreased ATP potency. B, At F291C, MTSEA (1 mm) application increases ATP potency. C, At R292C, ATP potency was decreased by overnight incubation with MTSEA (1 mm). D, Overnight incubation with MTSEA (positive charge, 1 mm) increased ATP potency at K309C, whereas overnight incubation with MTSES (negative charge, 1 mm) abolished the response. E, F, At A323C and I328C, ATP potency is unaffected by incubation with MTSES and MTSEA, respectively (1 mm for each; n = 3–6). Data are shown for overnight incubations with MTS reagent that gave the same results as bath-applied MTS reagents. Error bars indicate SEM.