Figure 3.
A, B, Higher magnification of synaptic terminals in the molecular layers of vermal cerebella (lobe IX). Arrows indicate postsynaptic density (psd), synaptic vesicle (sv), synaptic cleft (sc), and presynaptic dense projections (pdp). PD, Purkinje cell dendrite. Scale bars, 500 nm. C, D, Quantitative electron microscopic analysis of Purkinje dendritic synapse density of newborn WT mice treated with vehicle, EB, tamoxifen, or EB plus tamoxifen. C, Axospinous synapse density. D, Shaft synapse density. Ultrathin sections (60 nm in thickness) containing calbindin-immunoreactive Purkinje dendrites in lobe IX at 6 d of age were analyzed. Each column and error bar represent the mean ± SEM (n = 5 in each group). *p < 0.05 and **p < 0.01 versus vehicle; †p < 0.05 and ††p < 0.01 EB or tamoxifen versus EB plus tamoxifen; ☆☆p < 0.01 EB versus tamoxifen (by one-way ANOVA, followed by Duncan's multiple range test).