Figure 1.
Na,K-ATPase isoforms are reduced in gene-targeted mice. A, Reduced Na,K-ATPase α3 isoform mRNA expression in embryonic E18.5 d brains from WT, heterozygous (+/−), and homozygous (−/−) α3 knock-out mice by Northern blot. L32 was used as a loading control. Twenty micrograms of total RNA were loaded per lane. B, RT-PCR analysis of RNA from WT, heterozygous (+/−), and homozygous (−/−) α3 knock-out mice shows larger RNA transcript in −/− (arrow) but not +/− or WT mice. C, Western blot analysis of Na,K-ATPase isoforms in whole tissue extracts from hippocampus of adult male α1+/−, α2+/−, and α3+/− mice. Total protein loaded per lane was as follows: 10, 0.5, and 1 μg for α1, α2, and α3 isoform expression, respectively. D, Semiquantitation by densitometry on whole tissue extracts from adult hippocampus of α1+/−, α2+/−, and α3+/− mice shows reduction in α1, α2, and α3 isoforms, respectively. *p < 0.05 versus WT.