Figure 3.
Hdh
(CAG)150 mice require more time to transverse the balance beam. Mice were placed on a lit platform and traversed beams of different diameters (5 mm square and 11 mm round) to reach a safe chamber on the far side. Mice were tested at 20, 40, 50, 70, and 100 week time points.
a
, Eleven millimeter round beam (70 weeks);
b
, 11 mm round beam (100 weeks);
c
, 5 mm square beam (70 weeks);
d
, 5 mm square beam (100 weeks). Hdh
(CAG)150 mutant mice show no motor abnormalities up to and including 50 weeks of age (see supplemental Fig. 2, available at www.jneurosci.org as supplemental material).
a
,
c
, Although nonsignificant, at 70 weeks both HET and HOM mice required more time to traverse the 11 and 5 mm beams compared with WT mice (11 mm beam, F
(2,19) = 1.38; 5 mm beam, F
(2,18) = 2.4; effect for genotype, p > 0.05).
b
,
d
, At 100 weeks, both HET and HOM mice required significantly more time to traverse the balance beams compared with WT mice (11 mm beam: WT, 6.09 ± 0.78; HET, 9.08 ± 1.69; HOM, 13.92 ± 1.51 s; p < 0.05; 5 mm beam: WT, 10.11 ± 1.37; HET, 16.77 ± 1.78; HOM, 18.75 ± 0.76 s; p < 0.05). *p < 0.05, significance compared with WT; **p < 0.05, significance compared with HET. Data are expressed as mean ± SEM in seconds.