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- supplemental material - Supplemental Figure 1 The incremental activation of multiple axons and the separation of single-axon EPSCs. A, a series of EPSCs recorded from an E12 neuron when the stimulation pulse duration was varied systematically between 10-200 μs at 10 μs interval. This protocol was repeated 4X. The inset plots the amplitude of the NMDA-R EPSC against the pulse duration, and the dash horizontal lines represent discrete components of EPSC amplitude. Each vertical jump in the level is presumed to be the result of the activation of an additional axon. B, Similar experiments in an E14 neuron revealed only two components of NMDA-R EPSC amplitude. The arrowhead points to a late-arriving EPSC riding on top of the smaller EPSC, and its existence confirms the larger EPSC was the result of linear summation of two EPSCs. C, only a single level of EPSC was found in an E16 neuron. D, despite variation in the number of inputs found within each age group, significant reduction in the mean was seen between each age group (Kolmogorov-Smirnov test). The means for each age group are indicated by a vertical dashed line and are 3.1�0.3 (E12), 1.9�0.2 (E14-15), and 1.2�0.1 (E16).