Figure 2.
Effects of dopamine stimulation on cell surface levels of DRD2 in DTNBP1 siRNA transfected SH-SY5Y cells. A, Representative data from flow cytometric analysis of cell surface DRD2. The overlaid histogram shows relative fluorescence intensities of DRD2 from cells treated with random siRNA (black), random siRNA plus dopamine (DA; green), DTNBP1 siRNA (pink), or DTNBP1 siRNA plus DA (blue). B, Dopamine-induced changes in cell surface DRD2 expression. The geometric mean of cell surface of DRD2 fluorescence was obtained by flow cytometric analysis. Values are expressed as a percentage of random siRNA transfected cells. Error bars represent means ± SD (n = 4). C–E, Representative confocal images of DRD2 expression in SH-SY5Y cells transfected with random siRNA (C, D) or DTNBP1 siRNA (E, F). C, E, Not treated. D, F, Treated with 10 μm DA for 30 min. Scale bar, 10 μm.