Figure 2.
Emx1-mediated Cdk5 conditional KO mice have a defect in neuroblast migration, demonstrated by BrdU labeling. A, C, E, G, One hour after BrdU pulse labeling. There was no significant difference in the density of BrdU+ cells between Cdk5 ECKO and control brains in either the SVZ (A, C; control, 88,319 ± 16,806 cells/mm3; Cdk5 ECKO, 70,817 ± 6089 cells/mm3; n = 3; p = 0.3829) or the OB (E, G; control, 4219 ± 1513 cells/mm3; Cdk5 ECKO, 5582 ± 1008 cells/mm3; n = 3; p = 0.4949). B, D, F, H, Five days after BrdU pulse labeling. B, D, The number of labeled cells retained in the SVZ was greater in the Cdk5 ECKO brains (D; 7380 ± 849 cells, n = 3) than in the controls (B; 4665 ± 401 cells, n = 3). F, H, The number of labeled cells in the OB was lower in the Cdk5 ECKO brains (H; 66,472 ± 6525 cells, n = 3) than in the controls (F; 119,071 ± 7569 cells, n = 3). A′–D′, Higher-magnification views of the portion of SVZ marked by rectangles in A–D. Scale bars: A–D, E–H, 200 μm; A′–D′, 50 μm.