Figure 5.
Effects of mitoNCX inhibitors on the post-tetanic Cares.
Aa
,
Ab
, The two superimposed CaTs were evoked at a large MFB by HFS (33 Hz, 5 s) under control conditions (black line) and in the presence of 2 μm TPP+ (
a
, gray line) or 10 μm CGP37157 (
b
, gray line).
Ac
, ∫Cares
dt values were calculated from CaTs evoked by HFS (25 Hz or 33 Hz for 5 s), and mean values for the ratio of ∫Cares
dt in the presence of mitoNCX blocker (2 μm TPP+ or 10∼20 μm CGP37157) to that in control conditions were plotted in the bar graph. **p < 0.01
B
, Large MFBs were loaded with 50 μm OG5N together with 50 μm OGB1.
Ba
, The three superimposed ΔF/F
0 transients were evoked by 5 s HFS at different frequencies (10 Hz, 25 Hz or 33 Hz).
Bb
,
Bc
, Two superimposed ΔF/F
0 transients were evoked by HFS (33 Hz for 5 s) before (black line) and after bath application of 2 μm TPP+ (gray line,
Bb
) or 20 μm CGP37157 (gray line,
Bc
).
Bd
, Relative values for peak ΔF/F
0 (peak) and integrals of post-tetanic ΔF/F
0 (integ) in the presence of TPP+ or CGP37157 normalized to those under control conditions.
C
, Experiments similar to A were done at small en passant MFBs. The two superimposed CaTs were evoked by HFS (33 Hz, 5 s) before (black line) and after application of 2 μm TPP+ (gray line).
D
, Mean values for ∫Cares
dt evoked by the same HFS (33 Hz for 5 s) at large and small en passant MFBs before and after bath application of 2 μm TPP+. Note that ∫Cares
dt at small MFBs is not different from that at large MFBs in the presence of mitoNCX blockers.