Figure 2.
Amygdala-projecting VTA DA neurons have short duration action potentials. A, Example APs collected from neurons that project to the AMYG, PFC, or NAc with the corresponding immunocytochemistry. Neurons were labeled by the retrograde marker DiI (red), filled with biocytin during the recording (green), and immunocytochemically identified as TH(+) (blue). B, The distribution of AP durations in confirmed TH(+) neurons (top), Ih(+), TH(−) neurons (middle), and Ih(−) neurons (bottom) for each projection target. Rectangles represent the 25th and 75th percentiles of each sample, the solid black vertical lines indicate the means, and the broken gray line at 1.87 ms is the longest AP duration recorded in an NAc-projecting TH(−) neuron.