Figure 3.
Effects of MeβCD on GTRAP3-18 expression and GSH contents of CGNs. A, CGNs were treated with MeβCD at the indicated concentrations for 2 d. Immunoblot analysis was performed using each specific antibody. B, Protein bands were quantified against actin amounts in the same lysates. Results are presented as means of three independent experiments. C, CGNs were treated with 250 μm MeβCD for 2 d. Intracellular localizations of EAAC1 (green), GTRAP3-18 (red), and nuclei (blue) of CGNs were examined. D, CGNs were treated with 250 μm MeβCD for 2 d. After immunoprecipitation using anti-EAAC1 antibody, immunoblot analysis was performed. E, GTRAP3-18 levels were quantified against EAAC1 amounts in the precipitates. F, CGNs were treated with MeβCD at the indicated concentrations for 2 d. GSH was assayed using ThioGlo-1. Results are presented as means of three independent experiments. *p < 0.05 compared with control. G, After CGNs had been treated with 250 μm MeβCD for 2 d, they were triple stained using Hoechst33342 (blue), CMFDA (CMF; green), and anti-GTRAP3-18 antibody (Red).