Figure 1.
Microglia express the genes encoding Nox1- and Nox2-dependent NADPH oxidases. Ethidium bromide-stained agarose gels of reverse transcription-PCR products generated from Nox1, p22phox, NOXO1, NOXA1, Rac1, Rac2, Nox2, p40phox, p47phox, and p67phox mRNA in microglia purified by FACS from CX3CR1EGFP/+ mice or from Nox3, Nox4, Duox1, or Duox2 mRNA in FACS-purified CX3CR1EGFP/+ microglia (M), inner ear from P3 mice (IE), kidney from 16-d-old mice embryos (Kd), or adult mouse thyroid (Th). IE, Kd, and Th were from WT mice and were used as positive controls.