Figure 1.
A–D, MPTP-induced activation of p38 and JNK in the ventral midbrain and striatum. Animals were treated with a single dose of vehicle or MPTP and killed 1, 4, 12, and 24 h later or treated with a daily dose of MPTP for 8 d and killed on the ninth day. Representative immunoblots from ventral midbrain (MB) and striatum (ST) of animals treated with saline (C) and MPTP (lanes 1, 4, 12, 24 h, 8 d) depicting (A) the levels of phospho-p38 (pp38) and p38 and (B) JNK54, JNK46, phospho-JNK54 (pJNK54), phospho-JNK46 (pJNK46) in the MB and ST after MPTP treatment. C and D depict the level of JNK54, JNK46, phospho-JNK54 (pJNK54), phospho-JNK46 (pJNK46) in the MB and ST after 8 d of MPTP treatment. β-Tubulin levels were measured as loading controls. Densitometric analysis of the immunoblots representing the relative intensity of the immunoreactive bands from MB (n = 6) and ST (n = 3) are shown below the respective blots. They are represented as solid line for pJNK54 and JNK54, whereas pJNK46 and JNK46 are depicted as dotted lines in A and B. Activation of p38 MAPK and JNK is indicated by their respective pMAPK/MAPK ratio. The increase in ratio is expressed as fold increase with respect to the control ratio (1.0). Values are mean ± SD. Asterisks indicate values significantly different from corresponding control (p < 0.05). Repeated measures of ANOVA followed by Dunnet's test was performed for A and B, whereas paired t test was performed for C and D.