Figure 3.
Histological analysis at 48 h after hypoxia–ischemia. Nissl stain (A–C), TUNEL stain (D–F), anti-MBP stain (G–L), and anti-OX42 stain (M–O) of the contralateral side (left column), HI-challenged and saline-injected (middle column), and HI/PAI-1-injected (right column) of rat brains at 48 h of recovery. Shown are representative images in nine animals of each group. Note the expansion of CC (delineated with red line) and columnar lesions (arrows) in saline-treated animal brains (B). Saline-treated brains also showed increased TUNEL staining (E), fragmentation of MBP-positive processes (H), and reduced MBP staining in the soma (K) of oligodendrocytes and infiltration of OX42-positive macrophage in the CC (N). The PAI-1-treated brains showed localized CC swelling and TUNEL signals (arrowheads in C, F). Ctx, Cerebral cortex; St, striatum; CA, Ammon's horn of hippocampus; DG, dentate gyrus. Scale bar: A–F, 250 μm; G–I, 100 μm; J–L, 20 μm; M–O, 50 μm.