Figure 7.
APP multimerization stimulated increased tyrosine, APP, and Src phosphorylation. HUVECs were treated for 30 min in serum-free media with or without 1 μg/ml IgG1 (isotype control) or 1 μg/ml 22C11 in the absence or presence of pretreatment and incubation with DMSO vehicle or 1 μm PP2. Cell lysates were quantified and their proteins were resolved by 10% SDS-PAGE and Western blotted (A) using antibodies recognizing pAPP, APP, pSrc, Src, and α-tubulin (loading control). Optical density (O.D.) of pAPP (B), pSrc (C), and pTyr (D) protein bands were normalized against their respective APP, Src, and α-tubulin bands, respectively. Optical density values were averaged from five independent experiments (±SD) (*p < 0.001 from IgG control).