Figure 4.
D2 receptors modulate corticostriatal release in 1-month-old YAC128 mice. A, Release halftimes for 1-month-old WT slices after cortical stimulation at 1, 10, and 20 Hz in the presence and absence of amphetamine in vitro (n = 89–425 puncta for each condition; *p < 0.05, ***p < 0.001, Mann–Whitney test). B, Compared with untreated slices (Veh), both the D2 receptor agonist quinpirole (Quin) and amphetamine (Amph) decreased FM1-43 destaining in slices from 1-month-old WT mice. The inhibitory effect of Amph was reversed by the D2 receptor antagonist sulpiride (Amph+Sulp). C, Distribution of mean t1/2 of release for destaining curves shown in B. n = 346, 194, 246, and 224 puncta for Veh, Quin, Amph, and Amph+Sulp, respectively. ***p < 0.001 compared with Veh, Mann–Whitney test. D, Individual terminal halftimes of release from averaged puncta shown in B. E, Release halftimes for 1-month-old YAC128 slices after cortical stimulation at 1, 10, and 20 Hz in the presence and absence of amphetamine in vitro (n = 62–353 puncta for each condition; *p < 0.05, ***p < 0.001, Mann–Whitney test). Curves for A and E were fit with a Hill equation. F, Compared with untreated slices (Veh), both the D2 receptor agonist Quin and Amph decreased FM1-43 destaining in slices from 1-month-old YAC128 mice. The inhibitory effect of Amph was reversed by addition of the D2 receptor antagonist Sulp (Amph+Sulp). G, Distribution of mean t1/2 of release for destaining curves shown in F. n = 284, 271, 285, and 277 puncta for Veh, Quin, Amph, and Amph+Sulp, respectively. ***p < 0.001 compared with Veh, Mann–Whitney test. H, Individual terminal halftimes from averaged puncta shown in F.