Figure 5.
Intensity of the AFI signal and area of excitation, after sciatic nerve electrical stimulation in rats with intraplantar vehicle or capsaicin injection. a, d, Images of background fluorescence of the dorsal surface of the spinal cord at T13 of a vehicle- (a) and capsaicin-injected rat (d), at 32 min after injection. b, e, Areas of excitation on the ipsilateral (yellow) and contralateral (purple) side, at 32 min after injection. c, Time course of the intensity of the AFI signal after injection, as a percentage of ΔF/F before injection (baseline), in square selections on the ipsilateral side at the L4–L6 spinal level. f, Mean areas of excitation at 32 min after injection, as a percentage of the areas before injection (baseline). Scale bar, 1 mm. Grayscale bar ranging from −0.75% (black) to +0.75% (white) of the 16 bit range. Error bars indicate SEM. *p < 0.05, **p < 0.01, using Bonferroni's correction only in c.