Figure 4.
Patient CSF treatment does not affect dendritic branching, spines, Bassoon cluster density, or cell survival.
a
, Hippocampal neurons transfected with the fluorescent protein, Tomato-td, imaged before and after 1 d of treatment with control or patient CSF, and traced with NeuronJ. Control (top) or patient (bottom) CSF treatment did not affect dendritic branching or complexity. Scale bar, 100 μm.
b
, Quantification of primary dendrite number.
c
, Quantification of primary dendrite length. All values are shown as mean ± SEM (n = 9 cells, 3 independent experiments; 1 patient, 1 control sample; Student's t test, p > 0.6).
d
, Sholl analysis of dendrite complexity before (white) and after (black) 1 d of control (left) or patient CSF (middle) treatment. Comparison of the difference before and after control and patient CSF treatment (right).
e
, Hippocampal neurons transfected with fluorescent protein, Tomato-td, and treated for 1 d with control or patient CSF. Control (top) or patient (bottom) CSF treatment did not affect dendritic protrusion density. Scale bar, 5 μm.
f
, Quantification of the density of dendritic protrusions (Student's t test, p > 0.3).
g
, Patient CSF treatment for 1 d does not affect Bassoon cluster density (linear regression analysis; R
2 = 0.005, p = 0.79). All values are mean ± SEM (n = 18 cells, 3 independent experiments; 12 patient, 2 control samples) (supplemental Table 1, available at www.jneurosci.org as supplemental material).
h
, Quantification of the density of dissociated hippocampal cells in vitro after 1 d treatment of control or patient CSF.
i
, Quantification of the percentage of TUNEL-positive neurons in vitro (apoptotic cells). These measures were not significantly different between control or patient CSF treatment [n = 30 fields (750 μm2), 4 independent experiments; 1 patient, 1 control sample; Student's t test, p > 0.6].