Figure 8.
Dopamine marker immunoreactivity in motor regions of MBP-hsyn Tg mice after GDNF infusion. To examine the effects of GDNF infusion on dopaminergic cells, TH immunoreactivity in the caudoputamen of saline-infused NTg mice (A), GDNF-infused NTg mice (B), saline-infused MBP-hsyn Tg mice (C), and GDNF-infused MBP-hsyn Tg mice (D) was measured and analyzed in E (n = 6; age, 8 months in each group). TH immunoreactivity was also measured in the substantia nigra of saline-infused NTg mice (F), GDNF-infused NTg mice (G), saline-infused MBP-hsyn Tg mice (H), and GDNF-infused MBP-hsyn Tg mice (I), and analyzed in J (n = 6; age, 8 months in each group). Levels of DAT were also measured by immunohistochemistry in the caudoputamen of saline-infused NTg mice (K), GDNF-infused NTg mice (L), saline-infused MBP-hsyn Tg mice (M), and GDNF-infused MBP-hsyn Tg mice (N), and measured and analyzed in O (n = 6; age, 8 months in each group). Scale bars: A–D, K–N, 200 μm; F–I, 50 μm. *Significant difference between saline-treated MBP-hsyn Tg mice and NTg (p < 0.05, one-way ANOVA and post hoc Fisher). #Significant difference between saline-treated MBP-hsyn Tg mice and GDNF-treated MBP-hsyn (p < 0.05, one-way ANOVA and post hoc Fisher). Error bars indicate SEM.