Figure 7. IFNγ regulates the generation of neurons, oligodendrocytes, and astrocytes. Size-matched undissociated IFNγ+/+, IFNγ−/−, and IFNγ-treated neurospheres were differentiated and stained for the neuronal marker βIII-tubulin (red), the oligodendrocyte marker O4 (green), and the astrocytic marker GFAP (blue). A–I, Representative images of individual IFNγ+/+ (A, D, G), IFNγ−/− (B, E, H), and IFNγ-treated (C, F, I) neurospheres stained for βIII-tubulin, O4, and GFAP. J, A merged image of A, D, and G. K, A merged image of B, E, and H. L, A merged image of C, F, and I. M, Quantification of the production of neurons, oligodendrocytes, and astrocytes in primary IFNγ+/+ and IFNγ−/− neurospheres. More neurons and oligodendrocytes but fewer astrocytes were generated from IFNγ−/− neurospheres than from IFNγ+/+ neurospheres. N, Quantification of the production of neurons, oligodendrocytes, and astrocytes in primary neurospheres with or without IFNγ treatment. IFNγ treatment significantly reduced the production of neurons and oligodendrocytes but increased the generation of astrocytes. Scale bar (in A): A–L, 100 μm. ***p < 0.001.