Figure 5. Expression of Ebfs in multiple retinal neuronal types and subtypes. A–X, Sections from P21 mouse retinas were double-immunolabeled with a pan-Ebf antibody and those against the indicated cell type-specific markers. Sections in (B, C, F, H–N, P–R) were also weakly counterstained with DAPI. There is colocalization between Ebfs and Pax6, Barhl2, calretinin, syntaxin, GLYT1, or GlyRa1/2 in ACs and/or RGCs (A–F) but no colocalization with Dab1, GABA, GAD65, TH, ChAT, or Isl1 in ACs (G–L). Ebfs are coexpressed with Isl1 or Brn3b in RGCs (L, M), with Chx10, Bhlhb5, or recoverin but not with PKC in bipolar cells (O–Q, S–U), with Lim1 or calbindin in horizontal cells (R, V–X); however, they are not coexpressed with GS in Müller cells (N). Arrows point to representative colocalized cells, and insets show corresponding outlined regions at a higher magnification. IPL, Inner plexiform layer; OPL, outer plexiform layer. Scale bar (in X): A, D, E, G, O, 19 μm; B, C, F, H–N, P–X, 16.7 μm.