Figure 2. SERT function and expression is the same in mice subjected to 0 or 14 d of repeated swim. A, B, As expected, fluvoxamine inhibited 5-HT clearance time (A) and rate (B) in hippocampus of control mice (no swim); however, these effects of fluvoxamine were equivalent in mice exposed to repeated swim. Compared with PBS vehicle, fluvoxamine significantly slowed 5-HT clearance time and rate in both groups of mice (2-way ANOVA with Bonferroni post hoc test, *p < 0.05; **p < 0.01, ★p < 0.01, n = 5–9/group/dose). C, In a behavioral readout of SERT and NET function, repeated swim did not affect the response to SERT and NET blockers in the tail suspension test. Immobility was sampled every 5 s over a 6 min test. Compared with saline, fluvoxamine (30 mg/kg), fluoxetine (15 mg/kg), and DMI (15 mg/kg) reduced immobility time in the TST in both groups of mice equally, regardless of swim. Data are expressed as a percentage of respective saline control (2-way ANOVA with Bonferroni post hoc test, ★p < 0.01).