Figure 1. A–D, Cr2-cre-controlled EGFP reporter gene is expressed in NPCs of the subgranular zone. Representative images of EGFP-expressing cells in the spleen (A), the hippocampus (B), and the dentate gyrus (C, D) of a 2-month-old double-transgenic Cr2-cre;mT/mG reporter mouse. Ubiquitously expressed, membrane-targeted tandem dimmer Tomato is replaced by membrane-targeted EGFP (mG) after cre-dependent recombination. A, Representative fluorescent microscopy images of the spleen labeled with an anti-Cr2 (CR2/CD35, clone 7E9) antibody (red), EGFP (green), or Tomato (blue). B, Representative fluorescent microscopy images of the hippocampus stained with anti-Sox2 (red), EGFP (green), and Tomato (blue) from a Cr2-cre;mT/mG reporter mouse at low magnification. The merged image shows hippocampal cells expressing EGFP. C, High-magnification images of the boxed area in B (merged image) and three-dimensional reconstruction of a stack of deconvoluted confocal images. D, Orthogonal view of confocal microscopy images showing colocalization of membrane-targeted EGFP and Sox2 (arrow), which is an NPC marker in the subgranular zone. Scale bars, 300 μm.