Figure 1. Aβ25–35 increases the Et uptake in cultured microglia, astrocytes and neurons in a concentration- and time-dependent manner. A–C, Time-lapse measurements of Et uptake in cultured mouse microglia (A) and astrocytes (B) and in enriched cultures of neurons (C) under control conditions (white circles) or treated for 72 h with 10 μm Aβ25–35 (black circles). La3+ (200 μm), a connexin hemichannel blocker, applied at ∼10 min of Et uptake measurements decreased uptake. D–F, Averaged data normalized to control (dashed line) of Et uptake ratio of microglia (D), astrocytes (E), or neurons (F) treated for 24, 48, or 72 h with 0.1 (white bars), 1 (black bars), or 10 μm (gray bars) Aβ25–35. *p < 0.05, **p < 0.005, and ***p < 0.001, treatments compared with respective controls. G, Averaged data normalized to the effect induced by 72 h treatment with 10 μm Aβ25–35 on Et uptake ratio in microglia (left bars), astrocytes (middle bars), or neurons (right bars) exposed to the following Cx43 hemichannel blockers coapplied during dye uptake recording: La3+ (200 μm), Gap26 (200 μm), Gap27 (200 μm), and Cx43E2; or exposed to blockers of Panx1 hemichannels: Prob (500 μm), 10panx1 (200 μm), and E1b (200 μm). Also shown is the Et uptake rate in microglia and astrocyte cultures of Cx43−/− mice. *p < 0.05, **p < 0.005, and ***p < 0.001, treatments compared with maximal response induced by 10 μm Aβ25–35. Averaged data were obtained from at least six independent experiments. Error bars indicate SEM.