Trpv1 Reporter Mice Reveal Highly Restricted Brain Distribution and Functional Expression in Arteriolar Smooth Muscle Cells

The heat and capsaicin receptor, TRPV1, is required for the detection of painful heat by primary afferent pain fibers (nociceptors), but the extent to which functional TRPV1 channels are expressed in the CNS is debated. Because previous evidence is based primarily on indirect physiological responses to capsaicin, here we genetically modified the Trpv1 locus to reveal, with excellent sensitivity and specificity, the distribution of TRPV1 in all neuronal and non-neuronal tissues. In contrast to reports of widespread and robust expression in the CNS, we find that neuronal TRPV1 is primarily restricted to nociceptors in primary sensory ganglia, with minimal expression in a few discrete brain regions, most notably in a contiguous band of cells within and adjacent to the caudal hypothalamus. We confirm hypothalamic expression in the mouse using several complementary approaches, including in situ hybridization, calcium imaging, and electrophysiological recordings. Additional in situ hybridization experiments in rat, monkey, and human brain demonstrate that the restricted expression of TRPV1 in the CNS is conserved across species. Outside of the CNS, we find TRPV1 expression in a subset of arteriolar smooth muscle cells within thermoregulatory tissues. Here, capsaicin increases calcium uptake and induces vasoconstriction, an effect that likely counteracts the vasodilation produced by activation of neuronal TRPV1.


Introduction
TRPV1 is a nonselective cation channel that is activated by noxious heat, protons, the vanilloid compounds capsaicin (CAP) and resiniferatoxin (RTX), and membrane-derived lipids, including anandamide (Caterina et al., 1997;Caterina and Julius, 2001). Studies of Trpv1 knock-out mice demonstrated a critical contribution of TRPV1 to the cellular and behavioral responses to noxious heat (Caterina et al., 2000;Davis et al., 2000). Although expression of TRPV1 was originally reported to be restricted to primary afferent nociceptors of the dorsal root ganglia (DRGs), trigeminal ganglia (TG), and nodose ganglia (Szallasi et al., 1995;Caterina et al., 1997;Tominaga et al., 1998), subsequent studies argued for a much wider distribution, both in the CNS and in non-neuronal tissues.
Expression of TRPV1 in the CNS has been reported using a variety of methods, including pharmacological characterization (Steenland et al., 2006), immunohistochemistry (Toth et al., 2005;Cristino et al., 2006), in situ hybridization (Mezey et al., 2000), radioligand binding (Acs et al., 1996;Roberts et al., 2004), and reverse transcription (RT)-PCR (Mezey et al., 2000). Despite this abundance of studies, the existence of TRPV1 in the brain remains a controversial topic, mainly because the extent and localization of TRPV1 varies considerably among studies, even those using very similar assays. For example, RTX binding studies reported results ranging from a lack of binding in the CNS (Szallasi et al., 1995) to widespread binding throughout the brain (Roberts et al., 2004). In part, the lack of consensus reflects the limitations of traditional approaches to determining gene expression, including variable sensitivity, poor signal-to-noise, and lack of specificity.
There is also controversy as to the functional contribution of TRPV1 in the CNS. For example, several groups reported alterations in hippocampal synaptic function in Trpv1 knock-out mice (Marsch et al., 2007;Gibson et al., 2008). However, these reports did not provide anatomical or biophysical evidence of TRPV1 expression in these cells. Furthermore, other groups found no effect of TRPV1 agonists on hippocampal synaptic function (Kofalvi et al., 2006;Benninger et al., 2008). These results illustrate the importance of definitively resolving the distribution of TRPV1. This is especially true because TRPV1 expression in the CNS has important implications for the potential side effects of TRPV1 agonists and antagonists being developed for the treatment of chronic pain.
Here we used gene targeting to modify the Trpv1 genetic locus and generated two lines of reporter mice, which allow for a highly sensitive readout of TRPV1 expression patterns. Consistent with our early reports (Tominaga et al., 1998), we demonstrate robust expression of TRPV1 in primary afferent neurons. However, with the exception of very low-level expression in a few discrete brain areas, most notably within and adjacent to the caudal hypothalamus, we could not confirm previous findings of a widespread TRPV1 distribution in the CNS. Calcium imaging, whole-cell recording, and in situ hybridization experiments demonstrated that the expression patterns revealed in the reporter mice reflected functional TRPV1 expression that is conserved across multiple mammalian species. Finally, the sensitivity and cellular resolution of our genetic marking strategy revealed functional TRPV1 expression in a subset of arteriolar smooth muscle cells (SMCs) in thermoregulatory tissues.

Animals and injections
Animal experiments were approved by the Institutional Animal Care and Use Committee and conducted in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals and the recommendations of the International Association for the Study of Pain.
Intrathecal injections were performed as described previously (Cavanaugh et al., 2009). For RTX injections, mice were anesthetized with 1.5% isoflurane and injected subcutaneously with escalating doses of RTX on consecutive days (30, 70, 100, and 200 mg/ml), followed 7 d later with 200 mg/ml. Histology was performed 7-10 d after this final RTX injection.

Placental alkaline phosphatase and lacZ detection
Adult mice (6 -8 weeks old) were perfused with 10 ml of HEPES-buffered saline followed by 20 ml of ice-cold 3.7% formaldehyde. Brain, spinal cord (SC), peripheral tissue, DRGs, and TG were dissected out, postfixed 3-4 h [for placental alkaline phosphatase (PLAP) staining] or 1.5 h [for nuclear lacZ (nlacZ) staining] at 4°C, and cryoprotected overnight in 30% sucrose. For brain and SC, 40 -80 m sections were processed as free-floating sections. For DRGs, TG, and tongue, 14 m cryostat sections were processed on slides. Ear, dura, and cremaster muscle were processed as whole mounts. PLAP and nlacZ staining was performed as described previously (Shah et al., 2004).

RT-PCR
Adult mice were perfused with 10 ml of PBS followed by 10 ml of RNA later (Ambion). TG, liver, bladder, and cremaster were dissected out and placed in RNA later on ice. For brain regions, 500 m brain sections were cut with a McIlwain Tissue Chopper into ice-cold PBS, and brain regions were microdissected out with sharp tissue forceps and put into RNA later on ice. To avoid contamination, separate forceps were used for dissection of each tissue/brain region.
RNA isolation was performed with TRIzol reagent (Sigma) according to the manufacturer's specifications. One microgram of each RNA was reverse transcribed with the SuperScript II Reverse Strand Synthesis System (Invit-rogen) using an oligo(dT) primer. Control reactions omitted reverse transcriptase. PCR amplification (94°C for 5 min; 35 cycles of 94°C for 30 s, 58°C for 30 s, 72°C for 1 min; 72°C for 10 min) was performed with 1 l of cDNA using the following primer pair: 5Ј-gtttgtggacagctacagtg-3Ј and 5Јgaagccacatactccttgcg-3Ј. Control PCR amplification (94°C for 5 min; 25 cycles of 94°C for 30 s, 58°C for 30 s, 72°C for 1 min; 72°C for 10 min) was performed with a glyceraldehyde 3-phosphate dehydrogenase (GAPDH) primer pair (5Ј-accacagtccatgccatcac-3Ј and 5Ј-tccaccaccctgttgctgta-3Ј) to ensure cDNA integrity. Amplification products were visualized on a 1% agarose gel with ethidium bromide and sequenced to confirm identity.

Mouse in situ hybridization
For in situ hybridization histochemistry (ISHH), mice were killed by decapitation under deep anesthesia. Brain and L4/5 DRGs were dissected out, rapidly frozen in powdered dry ice, and cut on a cryostat (5 m for DRG and 10 m for brain). Sections were thaw mounted onto slides and fixed in 4% formaldehyde, pH 7.4, for 20 min. ISSH was performed as described previously (Kobayashi et al., 2005) with an 35 S-labeled cRNA probe directed against bases 1155-1908 of the full-length TRPV1 cDNA (antisense or sense; 1 ϫ 10 7 cpm/ml). For autoradiography, sections were coated with Kodak Nuclear Track Emulsion Type NTB (Carestream Health) diluted 1:1 with distilled water at 45°C and exposed for 2 months in lighttight boxes at 4°C. After development in D-19 (Carestream Health) and fixation in 24% sodium thiosulfate, the sections were stained with hematoxylin-eosin (H&E), air dried, cleared in xylene, and coverslipped.
For combined immunohistochemistry with ISHH, mice were deeply anesthetized with sodium pentobarbital and perfused transcardially with 20 ml of phosphate buffer (PB), followed by 50 ml of 4% formaldehyde. DRGs were dissected out and postfixed in the same fixative at 4°C overnight, followed by immersion in 20% sucrose in PB at 4°C for 3 d. Tissue was frozen in powdered dry ice and cut on a cryostat at a 5 m thickness. Sections were preincubated in TBS containing 5% normal goat serum (NGS) for 30 min, followed by incubation in guinea pig anti-TRPV1 (1:1000; Julius Laboratory, UCSF) in TBS containing 5% NGS for 24 h at 4°C, washed in TBS, and processed for DAB immunohistochemistry as described previously (Tominaga et al., 1998). Sections were then fixed in 4% formaldehyde for 5 min and immediately processed for ISHH, as described above, but emulsion was stopped after 3 weeks.

Rat, monkey, and human in situ hybridization
Frozen human tissues (with postmortem delay of 7 h) were acquired from the Brain and Tissue Bank for Developmental Disorders (University of Maryland, Baltimore, MD). Monkey tissues were obtained from an adult male Macaca fascicularis (ITR Laboratories Canada). Rat brain was acquired from adult male Sprague Dawley rats (Charles River).
Brains were rapidly dissected, snap frozen at Ϫ40°C in isopentane for 20 s, and stored at Ϫ80°C. Frozen tissues were cryosectioned at 14 m and mounted onto Superfrost Plus slides (VWR Canada). Slides were stored at Ϫ80°C until further use.
Species-specific antisense riboprobes for rat, monkey, and human TRPV1 were transcribed in vitro using SP6 RNA polymerase (Promega) and radiolabeled with 35 S-UTP and 35 S-CTP (ϳ800 Ci/mmol; GE Healthcare). After transcription, the TRPV1 DNA templates were digested with DNase I (Promega) and subsequently purified using G-50 Sepharose microspin columns (GE Healthcare). The quality of labeled riboprobes was verified by polyacrylamide-urea gel electrophoresis and scintillation counting.
For ISHH, tissue sections were fixed with 4% paraformaldehyde, rinsed three times in 2ϫ SSC, equilibrated in 0.1 M triethanolamine (TEA), and treated with 0.25% acetic anhydride in 0.1 M TEA. After a rinse in 2ϫ SSC and dehydration through an ethanol series (50 -100%), hybridization was performed in buffer containing 75% formamide (Sigma), 600 mM NaCl, 10 mM Tris-HCl, pH 7.5, 1 mM EDTA, 1ϫ Denhardt's solution (Sigma), 50 g/ml denatured salmon sperm DNA (Sigma), 10% dextran sulfate (Sigma), 20 mM dithiothreitol, and [ 35 S]labeled cRNA probe (20 ϫ 10 6 cpm/ml) at 55°C overnight in chambers humidified with 75% formamide. After hybridization, slides were rinsed twice in 2ϫ SSC at room temperature, treated with 20 g/ml RNase IA in RNase buffer (25 mM NaCl, 5 mM Tris-HCl, pH 7.5, 0.5 mM EDTA, pH 8.0) for 45 min at 37°C, and washed to a final stringency of 0.1ϫ SSC at 70°C. Sections were then dehydrated and exposed to Kodak Biomax MR-2 film. After exposure to film, the slides were dipped in Kodak NTB2 emulsion and exposed for 8 weeks at 4°C before development and counterstaining with H&E. Film autoradiograms were digitized with a highresolution Microimager digital camera (Xillix) via the MCID image analysis system (Imaging Research). Bright-and dark-field photomicrographs of emulsion-dipped tissue sections were captured using a microscope (DMRBE/DM 4000B; Leica) equipped with a DFC490 digital camera (Leica). Digital images were transferred to Adobe Photoshop 7.0 for minimal image processing.

DRG calcium imaging
DRG neurons were isolated from 6-week-old mice, dissociated, and subjected to ratiometric calcium imaging the next day as described previously (Caterina et al., 2000). Cells were stimulated with 1 M CAP, followed by 150 mM K ϩ (HiK) Ringer's solution, fixed, and stained for nlacZ histochemistry. All neurons that responded to HiK solution were included for analysis.
Whole-cell recording. After recovery, slices were transferred to a submersion chamber on an upright Olympus BX51 microscope and perfused with ACSF, saturated with 95% O 2 /5% CO 2 . Enhanced yellow fluorescent protein-positive (EYFPϩ) cells in the supramammillary nucleus (SuM) and posterior hypothalamus were identified by epifluorescence microscopy and recorded with 3-5 M⍀ borosilicate glass pipettes, filled with an internal solution consisting of (in mM) 140 CsMeSO 3 , 10 HEPES, 10 EGTA, 2 NaCl, 2 Mg-ATP, 1 QX-314, 5 TEA-Cl, and 1 CaCl 2 , pH 7.3. After the formation of a seal and achieving whole-cell configuration, membrane potential was held in voltage-clamp mode at Ϫ60 mV, and stable baseline holding current was established. The bath solution was then switched to a control ACSF solution containing TTX (500 nM), picrotoxin (100 M), APV (100 M), and NBQX (10 M) for several minutes, followed by ACSF containing 10 M CAP for ϳ5 min, and then back to ACSF to wash out responses. In some cases, 20 M Ruthenium Red (RR; final concentration) was added directly to the bath.
Ca 2ϩ imaging of acutely dissociated cells. Coronal slices of caudal hypothalamus were cut as described and transferred to a dissociation solution consisting of 2.5 mg/ml protease XXIII (Sigma) dissolved in the sucrose cutting solution. Slices were incubated at 35°C for 10 min, rinsed several times in cold sucrose solution, and placed in cold (4°C) sucrose solution containing 1 mg/ml trypsin inhibitor (Sigma) and 1 mg/ml bovine serum albumin (Sigma). The SuM was isolated with iridectomy scissors and  gently triturated using a series of fine-bore Pasteur pipettes. Dissociated neurons were imaged as described for DRG neurons.

Hippocampal slice imaging
Two hundred micrometer coronal slices of P19 hippocampus were cut from wild-type mice as detailed above for caudal hypothalamic slices. Slices were loaded with 100 m Fura-2-AM (Invitrogen) and 0.2% pluronic acid at 22-25°C for 60 min in ACSF, saturated with 95% O 2 /5% CO 2 , and calcium imaging was performed as detailed above for hypothalamic slices.

Arteriole calcium imaging
Third-order arterioles were dissected out of ears, bathed in ice-cold physiological salt solution (in mM: 137 NaCl, 5.6 KCl, 1 MgCl 2 , 0.42 Na 2 HPO 4 , 0.44 NaH 2 P0 4 , 4.2 NaHCO 3 , 10 HEPES, and 1 mg/ml BSA, pH 7.4), cut into ϳ2 mm pieces, and cultured in poly-D-lysine-coated wells in low-glucose DMEM with 10% fetal bovine serum and penicillinstreptomycin. The next day, arterioles were subjected to ratiometric calcium imaging as described above. Cells were stimulated with 1 and 10 M CAP, followed by 10 M RR to block responses, and finally with HiKϩ Ringer's solution.

TRPV1 reporter mice
We used gene targeting to create a line of mice (TRPV1 PLAP-nlacZ ) in which PLAP and nlacZ are expressed under the control of the TRPV1 promoter. We inserted an IRES-PLAP-IRES-nlacZ cassette immediately 3Ј of the TRPV1 stop codon, which permits the transcription and translation of PLAP and nlacZ in cells expressing TRPV1, without disrupting the Trpv1 coding region (Fig.  1A). In addition to providing a very sensitive detection method, background levels of PLAP and nlacZ are negligible in the mouse (Shah et al., 2004), which allows for discrete localization of TRPV1.
As expected, TRPV1 PLAP-nlacZ mice robustly expressed nlacZ in nuclei of primary afferent neurons of the DRG and TG (Fig.  1 B). PLAP histochemistry labeled both cell bodies and axonal processes in the DRG (Fig. 1C), as well as primary afferent terminals of the SC dorsal horn (Fig. 1 D). The SC staining was eliminated by intrathecal injection of high-dose CAP (data not shown), which selectively destroys TRPV1 fibers (Cavanaugh et al., 2009), indicating that the PLAP staining indeed arises from TRPV1 nociceptors.

nlacZ specifically marks CAP-responsive, TRPV1؉ DRG neurons
To establish the specificity of nlacZ expression for TRPV1 neurons, we investigated the CAP responsiveness of nlacZϩ DRG neurons cultured from adult TRPV1 PLAP-nlacZ mice, using livecell calcium imaging (Fig. 1 F-J ). Importantly, we found that Ͼ99% of nlacZϩ neurons responded to 1 M CAP and Ͼ90% of CAP-responsive neurons were nlacZϩ (n ϭ 568). We also compared the expression of nlacZ and TRPV1 in DRG sections (Fig.  1 E). Consistent with the calcium imaging results, we found that Ͼ95% of TRPV1-immunoreactive neurons were nlacZϩ. These results demonstrate that nlacZ histochemistry provides a sensitive and accurate correlate of functional TRPV1 expression, in vivo as well as in vitro.

Highly restricted expression of TRPV1 in the adult brain
We found no PLAPϩ cell bodies in the brain of TRPV1 PLAP-nlacZ mice. We did, however, find limited nlacZ expression in a few brain areas, primarily along midline structures of the rostral midbrain and caudal hypothalamus (Fig. 2). Prominent among these brain areas were the intrafascicular nucleus (IF) (Fig. 2 A) and SuM (Fig. 2 D), as well as the dorsomedial hypothalamus (DMH) and posterior hypothalamus (PH) (Fig. 2G). We also detected scattered nlacZϩ cells in the entorhinal cortex (Fig. 2 B), the rostral linear raphe nucleus (RLi) (Fig. 2C) and the periaqueductal gray (Fig. 2 E). Finally, nlacZ expression marked a discrete subset of (nonpyramidal) hippocampal neurons (Fig. 2 F) that colabeled for reelin, a marker of Cajal-Retzius cells (Fig. 2 F, inset), as well as reelinϩ cells in the accessory olfactory bulb (AOB) (Fig. 2 H). It is likely that our failure to detect PLAP in these brain regions reflects the greater sensitivity of nlacZ versus PLAP histochemistry.
We used several complementary approaches to verify the expression pattern revealed in the Trpv1 knock-in mice. We could not detect staining in any of these brain areas using TRPV1 antisera, most likely because TRPV1 is expressed at levels below the detection threshold of immunohistochemistry. Furthermore, with the exception of the caudal hypothalamus (which included the SuM and IF), we failed to detect TRPV1 mRNA by RT-PCR in any of the brain regions where we saw nlacZ expression (see Fig.  5J ). Finally, radioactive in situ confirmed the restricted expression suggested by the Trpv1 reporter mice, with positive signals in the IF, SuM, DMH, and PH (Fig. 3M-O). Together with the lack of PLAP staining, these results indicate that TRPV1 in the brain is limited to very low-level expression in a small subset of cells of a few discrete brain regions.

Restricted expression of TRPV1 in CNS is conserved across species
The distribution of TRPV1 in the DRG of the mouse differs from that of the rat (Woodbury et al., 2004), which suggests that brain expression patterns might also differ across species. To investigate this, we performed radioactive in situ hybridization in several species, including rat, monkey, and human. Importantly, using several different high-affinity probes directed against rat TRPV1, we found a near-identical pattern of CNS staining compared with that obtained in the mouse. Most notably, specific signals were found in caudal hypothalamic regions, including the PH, SuM, and IF ( Fig. 3E-L). Positive signals were also found in the dorsal motor nucleus of the vagus (DMV), mesencephalic trigeminal nucleus, parabrachial nucleus, RLi, and lateral hypothalamus of the rat (data not shown). In monkey (data not shown) and human brain sections ( Fig. 3R-S), we could not detect any TRPV1ϩ signal; however, we did not have access to sections that included the areas of the caudal hypothalamus that had positive signals in rat and mouse. Nevertheless, the lack of signal in other brain areas, despite the strong specific signal in DRG neurons (Fig. 3Q), is consistent with the highly restricted pattern we observed in mouse and rat, thus demonstrating that this property is conserved across multiple mammalian species.
Transient embryonic TRPV1 expression in the brain As this highly restricted CNS expression pattern contrasted with reports of widespread TRPV1 distribution, we created a second line of reporter mice (TRPV1 Cre mice), in which Cre recombinase is expressed under the control of the TRPV1 promoter ( Fig.  4 A). Crosses of this line with Cre-dependent reporter lines provide a highly sensitive fate map of TRPV1 expression, which reveals all loci of TRPV1 expression, no matter how transient. When we crossed TRPV1 Cre mice with the Rosa-lacZ reporter line (TRPV1 Cre /R26R-lacZ) (Soriano, 1999), we observed the expected pattern of DRG and SC staining (Fig. 4 B, C).
Importantly, all the nlacZϩ brain areas in the TRPV1 PLAP-nlacZ mice were also stained by lacZ in the TRPV1 Cre /R26R-lacZ mice (Fig. 4 D-K ). The staining in TRPV1 Cre /R26R-lacZ mice was more widespread than that in TRPV1 PLAP-nlacZ mice, with the exception of the reelinϩ cells of the hippocampus and AOB, likely because expression of the Rosa locus is limited in these cells (data not shown). The more extensive labeling in the TRPV1 Cre / R26R-lacZ mice compared with TRPV1 PLAP-nlacZ shows that the cellular distribution of TRPV1 in these brain areas undergoes a developmental restriction. We additionally noted several regions in which expression is observed in the TRPV1 Cre /R26R-lacZ mice, but not in the TRPV1 PLAP-nlacZ mice (Fig. 5). These are likely areas where TRPV1 is transiently expressed during the embryonic or early postnatal periods. However, because only a few molecules of Cre are necessary to induce recombination, it is possible that some of these areas represent regions where TRPV1 is expressed at extremely low levels in the adult, below the detection threshold of the TRPV1 PLAP-nlacZ mice. This could be the case for the DMV, where we observed in situ signals in both the mouse and rat despite a lack of staining in sections from TRPV1 PLAP-nlacZ mice (Fig. 3 H, L,P).

Functional expression of TRPV1 in caudal hypothalamic nuclei
As lacZ expression in the IF, SuM, DMH, and PH of reporter mice was confirmed by RT-PCR and radioactive in situ, we sought to validate functional TRPV1 expression in these brain areas using calcium imaging and whole-cell recordings in brain slices from TRPV1 Cre mice crossed to EYFP reporter mice (TRPV1 Cre / R26R-EYFP mice) (Srinivas et al., 2001). Importantly, EYFP staining in these mice was identical to lacZ expression in TRPV1 Cre /R26R mice (data not shown).
We performed calcium imaging experiments in caudal hypothalamic neurons in intact slices (Fig. 6 A-D) and acutely dissociated cells (Fig. 6 E-H ). We consistently observed calcium responses in EYFPϩ cells of hypothalamic slices in response to bath application of 10 M CAP, in the presence of the fast synaptic transmission blockers picrotoxin, NBQX, and APV and the voltage-gated sodium channel blocker TTX. In acutely dissociated cells, CAP elicited robust responses at doses as low as 100 nM. CAP responses were blocked by preincubation with 10 M RR, a nonselective antagonist that blocks many calcium-permeant ion channels including TRPV1 (Fig.  6 J), and were absent in Trpv1 knock-out mice (data not shown). CAP-induced calcium increases were limited to EYFPϩ cells; ϳ97% (30 of 31) of CAP-responsive cells were EYFPϩ. However, only ϳ59% (30 of 51) of EYFPϩ cells tested were CAP responsive, consistent with a developmental downregulation of TRPV1.
Finally, we performed whole-cell patch-clamp recordings from acute hypothalamic slices, containing the SuM and PH, prepared from P21-P26 TRPV1 Cre /R26R-EYFP mice. We performed voltage-clamp recordings from visually identified EYFPϩ neurons, held at a membrane potential of Ϫ60 mV, while bath applying 10 M CAP in the presence of TTX and synaptic blockers. We found that ϳ53% (10 of 19) of EYFPϩ neurons responded to CAP with inward current greater than Ϫ5 pA; the average amplitude was Ϫ52.9 Ϯ 14 pA (n ϭ 10) (Fig. 6 K). CAP responses generally desensitized during application, slowly re-versed after washout, and could be blocked by RR (data not shown).
As an additional test of the accuracy and selectivity of our reporter mice, we performed calcium imaging experiments in regions of the hippocampus where others have argued for a functional contribution of TRPV1 (Marsch et al., 2007;Gibson et al., 2008, Chavez et al., 2010, but where we see no anatomical evidence for TRPV1 expression. In intact slices of the dentate gyrus, we never observed calcium increases in response to administration of 10 M CAP (Fig. 6 L-O). We also failed to detect CAP responses in the CA1 region of the hippocampus (data not shown), although pyramidal cell loading in these preparations was suboptimal. However, we did not observe CAP-induced calcium increases in embryonic day 19 rat hippocampal cultures, which included CA1 and CA3 pyramidal cells (data not shown). These results are consistent with our anatomical data and demonstrate that the lack of reporter Figure 5. Developmental expression of TRPV1 in the brain. Left, lacZ staining in 80 m sections of TRPV1 Cre /R26R-lacZ mice. Right, Lack of nlacZ staining in the same areas in TRPV1 PLAP-nlacZ mice, likely attributable to developmental expression that is extinguished in the adult. A-I, Regions include the 10th and 12th motor nuclei of the medulla (A) (arrowheads indicate cell body staining in 10th and 12th motor nuclei; arrows indicate axonal lacZ label in the area postrema and nucleus of the solitary tract), nucleus interpolaris (B), perivolivary region (C), raphe magnus (D), central gray region (E), inferior colliculus (F), parabrachial nucleus (PB; arrowheads) and nucleus of the lateral lemniscus (arrows) (G), lateral hypothalamic nucleus (H, arrowheads), and paraventricular hypothalamic nucleus (PVH; I). J, RT-PCR analysis of brain regions and peripheral tissues to detect presence of TRPV1 mRNA. Note the faint band in caudal hypothalamus lane (arrow). OB, Olfactory bulb; Co, cortex; Hi, hippocampus; AH, anterior hypothalamus; CH, caudal hypothalamus; PG, periaqueductal gray; Ce, cerebellum; Li, liver; Bl, bladder; Cr, cremaster; neg, no cDNA. Scale bar, 500 m.
molecule expression parallels what, in our hands, is a functional absence of TRPV1.

Functional TRPV1 expression in arteriolar SMCs
Unexpectedly, when examining peripheral tissue for PLAPϩ axons, we observed discrete, striated staining running in proximity to blood vessels (Fig. 7A,C,E,F). We found comparable patterns of nlacZ staining (Fig.  7B,D,G), demonstrating that the PLAP derived from TRPV1ϩ cells that are intrinsic to these tissues. In agreement with this observation, RT-PCR analysis revealed TRPV1 mRNA in cremaster muscle, which has extensive nlacZϩ labeling (Fig. 5J ). Furthermore, the nlacZ staining colocalized with immunostaining for ␣-SMA, a filament that is specifically associated with SMCs (Fig. 7D).
The SMC label was not ubiquitous but was concentrated in thermoregulatory tissues, including the cremaster muscle ( Fig.  7 A, B), dura (Fig. 7C), tongue (Fig. 7D), trachea (Fig. 7E), skin (Fig. 7F ), and ear (Fig. 7G). In contrast, we found minimal to no SMC labeling in many other organs, including the liver, lung, kidney, pancreas, SC, brain, and aorta. SMC expression was restricted to certain small-to mediumdiameter vessels (Ͼ100 m in diameter), which, given their size and location, are most likely arterioles. Importantly, the SMC label was substantially reduced after systemic RTX, which kills TRPV1ϩ cells (Fig.  7G,H). In addition, we saw similar patterns of blood vessel staining in TRPV1 Cre /R26R-lacZ mice. (Fig. 7I,J).
As an additional test of the functionality of the TRPV1 expression in SMCs, we performed live-cell calcium imaging (Fig. 8A-F). Pieces of arteriole were dissected out of the ear and cultured overnight to ensure that any vessel-associated nerves were rendered nonfunctional during imaging. CAP (1 and 10 M) evoked arteriole constriction that was accompanied by increases in intracellular calcium (Fig. 8C,G,I). Importantly, RR (10 M) reversed the calcium responses (Fig. 8G,I), and neither calcium influx nor constriction was recorded in Trpv1 knockout mice (Fig. 8D,H,I). We conclude that TRPV1 is functional and mediates vessel constriction in arteriolar SMCs.

TRPV1 in the CNS
To unequivocally resolve the disagreement as to the extent of TRPV1 expression outside of the primary afferent nociceptor, we used a genetic approach to label TRPV1ϩ cells with excellent sensitivity and precision. In contrast to previous reports, we conclude that TRPV1 in the CNS is limited to very low-level expression in a few discrete brain regions. Most prominent among these is a contiguous band of cells centered along the midline of the posterior hypothalamus and rostral midbrain. We confirmed expression using a number of independent techniques, including RT-PCR, radioactive in situ hybridization, calcium imaging, and slice recording, thus providing multifaceted evidence that nlacZ in these brain areas represents bona fide TRPV1 expression.
We also performed in situ hybridization in rat, primate, and human tissue and found that the restricted expression pattern revealed by the Trpv1 reporter mice was conserved across these species. Notably, the areas of the caudal hypothalamus that have TRPV1ϩ in situ hybridization signals in both the mouse and rat are the only areas in the rat brain where injection of a high dose of CAP induces cell body degeneration (Ritter and Dinh, 1988), which demonstrates that these signals reflect functional TRPV1 expression in the rat, as we have shown for the mouse. The physiological function of TRPV1 in these brain areas is unclear; however, given the proximity of these regions to the third ventricle, it is possible that TRPV1 could detect changes in CSF temperature.
Several lines of evidence suggest that TRPV1 is present at extremely low levels in these brain areas. First, we were unable to detect TRPV1 by immunohistochemistry or PLAP staining. Second, we detected only a very faint TRPV1 band with by RT-PCR analysis and needed an extended (2 month) exposure period to observe signals with radioactive in situ. Finally, the average currents elicited by CAP in caudal hypothalamic neurons are ϳ40 times smaller than those observed in DRG neurons (Caterina et , 2000). Based on the single-channel conductance of TRPV1 at Ϫ60 mV (Tominaga et al., 1998), we estimate that the smallest CAP-induced currents (Ϫ5 pA) that we observed in EYFPϩ cells of the caudal hypothalamus arise from just a few functional channels, highlighting the remarkable sensitivity of the Trpv1 knock-in mice.
Several groups used Trpv1 knock-out mice to suggest a functional contribution of this channel outside of the DRG, including urothelial cells of the bladder (Birder et al., 2002), osmosensitive neurons of the supraoptic and paraventricular regions of the hypothalamus (Ciura and Bourque, 2006;Sharif-Naeini et al., 2006, 2008, striatal neurons (Maccarrone et al., 2008), dentate gyrus neurons (Chavez et al., 2010), medium spiny neurons of the nucleus accumbens (Greuter et al., 2010), and hippocampal pyramidal cells (Marsch et al., 2007;Gibson et al., 2008). However, despite using what we consider to be the most sensitive measure yet for detecting TRPV1, our analysis of Trpv1 reporter mice does not support the conclusions of these reports. We did not detect lacZ in any of these cell populations, even in the TRPV1 Cre / R26R-lacZ mice, where embryonic expression greatly exceeds that observed in the adult and in which only a few molecules of Cre expression are sufficient to drive recombination. In agreement with the lack of reporter molecule expression, CAP did not elicit calcium increases in either intact hippocampal slices or in cultured hippocampal neurons. Our observations are thus more consistent with studies that found no evidence for TRPV1 in several candidate regions (Kofalvi et al., 2006;Benninger et al., 2008;Taylor et al., 2008;Everaerts et al., 2010).
One caveat is that the use of the internal ribosome entry site in our reporter constructs could have led to spurious reporter molecule expression in areas that lack Trpv1 transcription, or to artificially low expression in certain brain areas that do express TRPV1. However, given the consistency of our results using multiple independent assays, and across various species, we do not believe this to be the case. It is also possible that our reporters are not expressed in cells that transcribe alternate isoforms of Trpv1 that lack the final exon, although such splice variants have not been reported. Finally, although many of the reports of functional TRPV1 in the brain argue for a postsynaptic localization, some of the phenotypes described in these knock-out papers could reflect a loss of presynaptic TRPV1 that arises from axonal projections of neurons that we demonstrate do express TRPV1. For example, SuM neurons are known to project heavily to the hippocampus (Vertes, 1992).

TRPV1 in arteriolar SMCs
Classic studies have shown that CAP-evoked release of neuropeptides from the peripheral nociceptor terminals promotes vasodilation. More recently, a direct action of CAP on vascular smooth muscle was reported, but whether this mechanism is widespread or regionally restricted has not been determined (Donnerer and Lembeck, 1982;Duckles, 1986;Kark et al., 2008). Although TRPV1 immunoreactivity has been observed in rat SMCs (Kark et al., 2008) we did not see similar staining in the mouse (data not shown), likely because of high nonspecific antibody staining in peripheral tissues. On the other hand, PLAP and nlacZ provided strong signals in SMCs with minimal background. Importantly, nlacZ staining was reduced in SMCs after systemic RTX injection, confirming functional TRPV1 expression. Finally, calcium imaging of isolated arterioles from the ear showed that genetically marked SMCs respond to CAP application, leading to arteriole constriction. Similar responses could not be evoked in Trpv1 knock-out mice, demonstrating that the direct CAP effects on SMCs require TRPV1.
Interestingly, the SMC labeling was restricted to a subset of arterioles in thermoregulatory tissues, including the tongue, skin, trachea, and cremaster. Whereas activation of TRPV1 on sensory nerve endings leads to local vasodilation, vascular TRPV1 mediates vasoconstriction. Thus, activation of TRPV1 on SMCs could counteract nerve-related changes in vascular tone in response to physiological TRPV1 agonists, such as heat and pH. TRPV1 expression in SMCs may therefore contribute to the thermoregulatory effects induced by TRPV1 antagonists, many of which are being developed to treat chronic pain conditions (Gavva, 2008). On the other hand, the limited TRPV1 expression in the brain indicates that untoward side effects of these drugs are unlikely to be CNS mediated.