Article Information
- Received September 13, 2010
- Revision received January 31, 2011
- Accepted February 4, 2011
- First published March 30, 2011.
- Version of record published March 30, 2011.
Author Information
- Dominik Herzog1,5,
- Pirmin Loetscher1,
- Jolanda van Hengel2,3,
- Sebastian Knüsel1,
- Cord Brakebusch4,
- Verdon Taylor5,6,
- Ueli Suter1, and
- João B. Relvas1,7
- 1Institute of Cell Biology, Department of Biology, Eidgenössische Technische Hochschule Zürich, CH-8093 Zürich, Switzerland,
- 2Molecular Cell Biology Unit, Department for Molecular Biomedical Research, Vlaams Instituut voor Biotechnologie, B-9052 Ghent, Belgium,
- 3Department of Biomedical Molecular Biology, Ghent University, B-9000 Ghent, Belgium,
- 4Biomedical Institute, Biotech Research and Innovation Center, University of Copenhagen, 2200 Copenhagen,
- 5Department of Molecular Embryology, Max Planck Institute of Immunobiology, D-79011 Freiburg, Germany,
- 6Centre for Stem Cell Biology, Department of Biomedical Science, University of Sheffield, Western Bank, Sheffield, S10 2TN, United Kingdom, and
- 7Instituto de Biologia Molecular e Celular, Universidade do Porto, 4150-180 Porto, Portugal
Author contributions
D.H., V.T., and J.B.R. designed research; D.H., P.L., and S.K. performed research; J.v.H. and C.B. contributed unpublished reagents/analytic tools; D.H., U.S., and J.B.R. analyzed data; D.H., V.T., U.S., and J.B.R. wrote the paper.
Disclosures
- Received September 13, 2010.
- Revision received January 31, 2011.
- Accepted February 4, 2011.
This work was supported by the Swiss National Science Foundation and by the National Center of Competence in Research “Neural Plasticity and Repair.” Research in the Taylor laboratory was supported by the Deutsche Forschungsgemeinschaft (TA-310-2 to V.T.), Landesstiftung Baden-Württemberg (ASII/29 to V.T.), and the Max Planck Society. Research in the Relvas laboratory was supported by Fundação para a Ciência e Tecnologia of Portugal Grant PTDC/BIA-BCM/112730/2009. We thank L. Sommer and N. Mantei for valuable advice and discussions and E. B. Crenshaw and P. Soriano for providing transgenic animals. We also thank the Light Microscopy Centre of the Eidgenössische Technische Hochschule Zürich for technical help with confocal imaging. The Islet-1 monoclonal antibody developed by T. M. Jessel was obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the National Institute of Child Health and Human Development and maintained by The University of Iowa, Department of Biology (Iowa City, IA). The Par3 antibody was a kind gift from I. Macara (Charlottesville, VA). The DN mDia1 expression plasmid mDia1 F2ΔN1 YFP was a kind gift from Dr. A. Alberts (Van Andel Institute, Grand Rapids, MI). The GST PAK/crib domain construct was provided by J. Collard (The Netherlands Cancer Institute, Amsterdam, The Netherlands).
The authors declare no competing financial interests.
- Correspondence should be addressed to João B. Relvas, Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal. jrelvas{at}ibmc.up.pt
Online Impact