Figure 4. Nuclear localization and firing pattern of the recorded relay cells. A, Localization of all juxtacellularly recorded cells in the thalamus in four coronal planes according to the rat brain atlas of Paxinos and Watson (1998). Blue, red, and green dots represent the position of the recorded VB, Po, and Border neurons, respectively. The green asterisk indicates the neuron shown in C–E. B, Single unit activity of representative VB (blue), Po (red), and Border (green) cells (marked by asterisks in A) and the simultaneously recorded LFP from the S1 cortex. Insets show burst events at a faster time scale. C, Neurolucida drawing of a border cell overlaid on the low-power image of the vGluT2 immunostained coronal section in which the cell body was found. The green trace in B shows juxtacellular recording of the same cell. The border region is indicated by two dashed lines in C. D, The inset of C (white rectangle) at higher magnification. Note the difference in vGluT2 immunostaining between Po and VPM. E, At high power, large vGluT2 terminals contact the proximal dendrites of the labeled cell (arrows). Asterisk = blood vessel. Scale bars: C and D, 200 μm; E, 20 μm.