Figure 1. Glial chain migration in a control wing and upon early/late ablation. A–D, Immunolabeling showing wing glia (green) and neurons (anti-22c10, red) at different stages: onset of migration (A), reaching level of the costa (B), completion of migration (C), 14 hAPF (D). In all panels, proximal is to the left, anterior to the top. E–H, In this and in following figures, snapshots show projections from time-lapses on repo>GFP wings. E–H correspond to the regions outlined in A and C, respectively. E, Before migration, glia show simple morphology and low GFP level (color-coded inset). F, By 17 hAPF, GFP intensity is higher (inset) and cells extend filopodia. The four double arrowheads (E,F) indicate the position of the ablated cells. G, By 22 hAPF, the migration front reaches the level of the costa. Open arrowheads show new glia appearing on the margin. H, The glial chain completes migration (arrow), joining radius glia. I, Graph representing migration efficiency in control wings and in wings in which the first four cells were ablated early or late (*p < 0,05, n = wing number). J–L show the raw data: J refers to migration initiation, K to reaching level of costa, and L to migration completion. The x-axis shows three experimental conditions: controls (gray circles), late-ablated wings (red circles), and early-ablated wings (blue circles). The y-axis indicates stages as hAPF. Each circle represents one sample; horizontal bars, average values. M, Ablation data summary. The first column indicates the experimental conditions, the second, the third and the fourth columns indicate the stage of achievement (hAPF) of migration initiation, reaching the level of costa and completion. For each column, from left to right: wing number, average, earliest and latest stages. Bars: A–C, 100 μm; D, 10 μm; E–H, 30 μm.