Figure 2. Developmental time and not behavioral state confers aldicarb hypersensitivity. A, Satiated worms (gray line) do not display significant acceleration relative to control worms (black line) in the time course to paralysis on aldicarb. B, Heat shocked wild-type animals (dashed black line, n = 25) displayed aldicarb-resistance relative to non-heat-shocked controls (solid black line, n = 16), as did the heat-shocked strain carrying an extrachromosomal array of hs::LIN3C (dashed gray line, n = 23) in comparison to non-heat shocked controls of the same strain (solid gray line, n = 26). C, During the adult stage, egl-4 loss-of-function (lf) mutants (dashed black line, n = 37) are aldicarb-resistant relative to adult wild-type (wt) worms (solid black line, n = 33). During the L4 lethargus (leth) stage, egl-4(lf) (dashed gray line, n = 24) are not different from wild-type worms (solid gray line, n = 21). D, During the adult stage, the aldicarb sensitivity of egl-4 gain-of-function (gf) mutants (dashed black line, n = 25) is not different from that of adult wild-type worms (solid black line, n = 27). During the L4 lethargus stage, egl-4(gf) (dashed gray line, n = 42) mutants are not different from wild-type worms (solid gray line, n = 22). E, During the adult stage, ceh-17 mutants (dashed black line, n = 30) show no difference from wild-type worms (solid black line, n = 23). During L4 lethargus, ceh-17 mutants (dashed gray line, n = 24) show no difference from wild-type worms (solid gray line, n = 29). F, nhr-25(ku217) mutants (dashed lines) were not different from wild-type worms (solid lines) during lethargus (gray lines, n = 29–34) or during the mid L4 stage (black lines, n = 31–33). G, The time course to paralysis on aldicarb during L4 lethargus of nhr-23I(RNAi)-treated animals (gray, n = 14) is not different from the time course of empty vector (ev) RNAi-treated animals (black, n = 21).