Figure 9. BAT-injected PRV infects preoptic glutamatergic neurons. A, B, PRV-614-infected cells (red) and VGlut2-GFP-expressing neurons (green) are shown at two levels of the preoptic hypothalamus (bregma +0.5 mm, left; +0.26 mm, right) after injection of the tracer virus into BAT of a VGlut2-GFP BAC transgenic mouse. C, D, The distribution of neurons containing GFP (green dots), PRV-614 (red dots), or both markers (blue stars) at these two levels is shown. High-magnification images taken from the outlined areas in A (E, G, I) and B (F, H, J) show VGlut2-GFP neurons (E, F) containing PRV-614 (G, H) in which the markers colocalize (I, J). In the high-magnification images (E–J), arrows indicate GFP/PRV-614 double-labeled cells. Scale bar (in A) A, B, 200 μm; (in I) E–J, 10 μm.