Figure 4.
Aberrant morphology of Purkinje cells in crmp5−/− mouse cerebellum at P21 and P28, but not P14. A–F, Sagittal sections of the crmp5+/− (A–C) and crmp5−/− (D–F) mouse cerebellum at P14 (A, D), P21 (B, E) and P28 (C, F) stained with anti-calbindin antibody. G, The Purkinje cells stained with anti-calbindin antibody. The length of long axis of the cell body (yellow line) and the width of the primary dendrites (red line) 20 μm away from the original point of the initial segment (blue line) were measured. H, Quantitative data of the length of long axis of the Purkinje cell soma at P14, P21, and P28. In crmp5−/−, size of the cell bodies became smaller at P21 and P28. I, Quantitative data of the width of the primary dendrites at P14, P21, and P28. In crmp5−/−, the width of the primary dendrites became thinner at P21 and P28. In all cases, three littermates of each genotype were used for quantification. *p < 0.01 compared with the corresponding value in crmp5+/− by Student's t test. Scale bar, 50 μm.