Article Information
- Received November 19, 2010
- Accepted December 17, 2010
- First published February 23, 2011.
- Version of record published February 23, 2011.
Author Information
- Bart G. Borghuis1,
- Lin Tian1,
- Ying Xu2,
- Sergei S. Nikonov2,
- Noga Vardi2,
- Boris V. Zemelman1, and
- Loren L. Looger1
- 1Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, Virginia 20147, and
- 2School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104
- Correspondence should be addressed to Bart G. Borghuis at the above address. borghuisb{at}janelia.hhmi.org
Author contributions
Disclosures
- Received November 19, 2010.
- Accepted December 17, 2010.
This work was supported by the Howard Hughes Medical Institute (B.G.B., L.T., B.Y.Z., and L.L.L.) and the National Institutes of Health (Y.X., S.S.N., and N.V.). Experiments were performed in the laboratory of Anthony Leonardo. Karel Svoboda generously provided the two-photon microscope and advised the project. We thank Vijay Iyer for technical assistance with the imaging system and scan software. OGB-1-AM was a generous gift from Luke Lavis. Thanks to Anthony Leonardo and Vivek Jayaraman for helpful discussion. We thank Amy Hu and Alma Arnold for assistance with histology. All affiliations are Howard Hughes Medical Institute, Janelia Farm. We also thank Jean Bennett and Albert Maguire (University of Pennsylvania) for introducing B.G.B. to the use of viral methods and intravitreal injection into the living mouse eye. All constructs and viruses used in this research are available from the University of Pennsylvania Vector Core (http://www.med.upenn.edu/gtp/vector_core.shtml). All experiments were conducted according to protocols approved by the Institutional Animal Care and Use and Institutional Biosafety Committees of the Howard Hughes Medical Institute, Janelia Farm Research Campus. B.G.B. and L.L.L. designed the research. B.G.B. performed all experiments. L.T. created GCaMP3 constructs. B.V.Z. created AAV vectors and constructed the synthetic mGluR1 promoter. N.V., Y.X., and S.S.N. electroporated retinas and assisted with bipolar cell recordings. B.G.B. and L.L.L. wrote the paper, with input from the other authors.
The authors declare no competing financial interests.
- Correspondence should be addressed to Bart G. Borghuis at the above address. borghuisb{at}janelia.hhmi.org
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