Figure 7.
GluRδ2 mRNA and promoter activity are detected highly in PCs and weakly in basket/stellate cells. A, Fluorescent in situ hybridization showing exclusive expression of GluRδ2 mRNA (green) in the cerebellum in the adult mouse brain. Note that sense probe yields no specific labeling (inset). B, Double-labeling fluorescent in situ hybridization for GluRδ2 (green) and GAD67 (red) mRNAs. In addition to heavy labeling in PCs (arrowheads), GAD67 mRNA-positive interneurons in the molecular layer (arrows) are consistently labeled for one of two tiny punctate signals for GluRδ2 mRNA. No such labeling is seen for GAD67 mRNA-positive interneurons in the granular layer (double arrowhead). C, D, Immunofluorescence for Cre recombinase in Grid2Cre/+ mice. No significant staining is observed in the brain of control mice (inset). E, F, Triple immunofluorescence for Cre recombinase (red), calbindin (blue), and parvalbumin (PV, green) in the molecular layer of Grid2Cre/+ mice. Intense immunolabeling for Cre recombinase is observed in the nucleus of calbindin/parvalbumin-positive PCs, while weak labeling is also detected in the nucleus of calbindin-negative/parvalbumin-positive interneurons in the molecular layer (arrows in E, F). G, No Cre recombinase (red) is detected in neurogranin (Ng, green)-positive Golgi cell (double arrow). OB, Olfactory bulb; Ctx, cerebral cortex; Mb, midbrain; Cb, cerebellum; ML, molecular layer; PCL, Purkinje cell layer. Scale bars: A, C, D, 1 mm; B, E–G, 20 μm.