Figure 3. Expression of additional HC and synaptic markers. Mice of the indicated genotypes received tamoxifen (TMX) at P0 and P1 and were analyzed at P22. A, B, Double staining of HA (white) and calretinin (red) in control (A) and experimental (B, B′) mice at P22. B is calretinin alone and B′ is the merged image. C–F′, Triple staining of HA (white), calbindin (green), and KCNQ4 (red) in control (C, D) and experimental (E–F′) mice at P22. E is KCNQ4 alone and E′ is the merged image. F is KCNQ4 alone and F′ is the merged image. E–F′ show the same cell visualized at confocal Y–Z plane (E, E′) and X–Y plane (F, F′) to better show the KCNQ expression pattern. G–L′, Costaining of α9 AChR-EGFP (green) and HA (white) in control (G) and experimental (H, H′) mice at P22. H is α9 AChR-EGFP alone and H′ is the merged image. Triple staining of HA (white), α9 AChR-EGFP (green), and CSP (red) in control (I) and experimental (J, J′) mice at P22. J is CSP alone and J′ is the merged image. Triple staining of HA (white), α9 AChR-EGFP (green), and synaptophysin (red) in control (K) and experimental (L, L′) mice at P22 is shown. L is synaptophysin alone and L′ is the merged image. All images were visualized in the confocal Y–Z plane, except D, F, and F′, which were imaged in the X–Y plane. Scale bars: 20 μm (A, C, G, I, K), 10 μm (B′, E′, H′, J′, L′), and 5 μm (D).