Figure 4. Injection of NMB induces edema and hyperalgesia and administration of NMB antagonist attenuates mustard oil-induced neurogenic inflammation. To determine the effects of NMB and BIM 23042, paw volume was measured by plethysmometry (a, d), thermal sensitization was assayed using the Hargreaves test (b, e), and mechanical hyperalgesia was quantified using von Frey microfilament measurements (c, f). Hindpaws were injected with NMB or NMB, SP, and CGRP as indicated (a–c). Pretreatment of mice with NMB antagonist BIM 23042, or BIM 23042, CGRP8–37, and spantide dramatically reduces mustard oil-induced swelling and hyperalgesia compared with vehicle-treated animals (d–f). Data represent means ± SEM; n ≥ 6 animals; significant differences between treatments (Student's t test) are indicated by *p < 0.05, **p < 0.01, and ***p < 0.001.