Article Information
- Received May 9, 2012
- Revision received July 6, 2012
- Accepted July 25, 2012
- First published September 12, 2012.
- Version of record published September 12, 2012.
Author Information
- Ferdinand Kaya1,*,
- Abdelkrim Mannioui2,3,4,*,
- Albert Chesneau1,
- Sowmya Sekizar2,3,4,
- Emmanuelle Maillard1,
- Chantal Ballagny1,
- Ludivine Houel-Renault9,
- David DuPasquier8,
- Odile Bronchain1,
- Isabelle Holtzmann1,
- Anne Desmazieres2,3,4,
- Jean-Léon Thomas2,3,4,7,10,
- Barbara A. Demeneix5,
- Peter J. Brophy6,
- Bernard Zalc2,3,4,7,*, and
- Andre Mazabraud1,*
- 1CNRS UPR 3294, Neurobiology and Development, Université Paris Sud XI, 91405 Orsay, France,
- 2Université Pierre-et-Marie-Curie-Paris 6, Centre de Recherche de l'Institut du Cerveau et de la Moelle épinière, 75013 Paris, France,
- 3Inserm UMR_S 975, 75013 Paris, France,
- 4CNRS UMR 7225, 75013 Paris, France,
- 5CNRS UMR 7221, Muséum d'Histoire Naturelle, 75005 Paris, France,
- 6Centre for Neuroregeneration, University of Edinburgh, Edinburgh EH16 4SB, United Kingdom,
- 7Assistance Publique-Hôpitaux de Paris, Groupe Hospitalier Pitié-Salpêtrière, 75013 Paris, France,
- 8WatchFrog, 91000 Evry, France,
- 9Centre Commun de Microscopie Electronique, Université Paris Sud XI, 91405 Orsay, France, and
- 10Department of Neurology, Yale School of Medicine, New Haven, Connecticut 06511
Author contributions
Author contributions: B.Z. and A. Mazabraud designed research; F.K., A. Mannioui, A.C., S.S., E.M., C.B., L.H.-R., D.D., I.H., and A.D. performed research; D.D. and P.J.B. contributed unpublished reagents/analytic tools; F.K., A. Mannioui, D.D., A.D., J.-L.T., B.D., P.J.B., B.Z., and A. Mazabraud analyzed data; O.B., J.-L.T., B.D., P.J.B., B.Z., and A. Mazabraud wrote the paper.
↵*F.K., A. Mannioui., B.Z., and A. Mazabraud contributed equally to this work.
Disclosures
- Received May 9, 2012.
- Revision received July 6, 2012.
- Accepted July 25, 2012.
This work was supported by the CNRS (B.D., A. Mazabraud, B.Z.), Inserm (B.Z., J.-L.T.), UPMC (B.Z., J.-L.T.), the Ville de Paris, Région Ile de France, Medicen, and the Ministry of Industry through the AMBRe consortium (B.D., B.Z. and A. Mazabraud). F.K. was supported by a fellowship from the Fondation ARSEP (Fondation pour l'Aide à la Recherche sur la Sclérose En Plaques) and A. Mazabraud by a fellowship from the Fondation pour la Recherche Médicale. P.J.B. was on sabbatical leave and supported by a grant provided by the Ecole des Neurosciences de Paris and the Wellcome Trust. We thank Dr. S. Nagata for the gift of monoclonal antibody against Xenopus MBP and Dr. A. Gow for providing us with the pMG2 plasmid. The Nkx2.2 antibody, developed by T. M. Jessell and S. Brenner-Morton, was obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the NICHD and maintained by the University of Iowa, Department of Biological Sciences, Iowa City, IA.
This paper is dedicated to the memory of the late Pr. David R. Colman and of Pr. Maurice Wegnez who initiated this work. We Are grateful to Dr. François Tiaho for introducing and encouraging us to use two-photon microscopy for live imaging of tadpoles, Aurelien Dauphin from our imaging facility, PICPS, for his precious help, Chistophe De Medeiros for animal care, and Muriel Perron for helpful discussions.
Dr. Barbara Demeneix is a founder of the company WatchFrog and Dr. David DuPasquier is an employee of WatchFrog.
- Correspondence should be addressed to Dr. André Mazabraud, CNRS UPR 3294, Neurobiology and Development, Université Paris Sud XI, Bâtiment 445, 91405 Orsay, France. andre.mazabraud{at}u-psud.fr
Online Impact